Estimation method for serial dilution experiments core. In this lab, a serial dilution will be made of a sample of bacteria, and then those dilutions will be used to culture bacteria in order to estimate their numbers. Serial dilution schemes in performing substrate and inhibitor titrations in enzyme activity assays, it is convenient to vary the concentration of the titrated ligand by a serial dilution scheme. Pdf estimation method for serial dilution experiments. There are many ways to evaluate health threats from toxic substances in the environment. Serial dilutions a serial dilution is any dilution where the concentration decreases by the same quantity in each successive step. Note that serial dilution is a special case of series of dilutions. Understand how stock solutions are used in the laboratory. Pdf titration of microorganisms in infectious or environmental samples is a corner.
File type pdf lab dilutions guide lab dilutions guide serial dilutions lesson a very quick lesson on how to do the math for serial and simple dilutions. While you may already make solutions in the lab by following recipes, we hope this webinar will help you understand the concepts involved so that you can. Oct 04, 2015 how to do a serial dilution 9ml test tubes mccc microbiology. This exercise helps students gain skill at performing dilutions without using reagents, bacterial cultures, or viral cultures, while being able to visualize the process. The source of dilution material for each step comes from the diluted material of the previous step example. Lab example for concentration and dilution this video discusses how to perform the calculations and procedure. A microbe is an organism that is microscopic and submicroscopic, which is too tiny to be viewed under a naked eye. Since a great deal of experimental lab work in biology involves solutions, the ability to work with and manipulate them is very important. In this exercise, you will learn the basic principles of spectrophotometry and serial dilution and their practical applications. Pdf lab report of microbiology akbar haqi academia. One common unit of concentration is molarity m, which is equal to moles of solute per liters of solution. Making serial dilutions objective create standard dilutions of a phage sample in order to perform plaque assays or titers. Serial dilutions austin community college district. In order to obtain a certain concentration of acid or base, dilutions are made.
A tour of the microbiology lab section one duration. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. The dilution of a sample in a diluent the liquid used. You will need these skills to complete other exercises throughout the semester. In the lab, correct dilutions of solutions are critical to performing accurate science.
Serial dilutions and viable cell count 1 serial dilution and viable cell count you go to the lake to swim, only to see a sign that says that there is no swimming. Booker microbiology 2310 lab report 3 serial dilutions october 15, 2017 introduction. A serial dilution is the dilution of a sample, in 10fold dilutions. Aids clinical trials group virology manual for hiv. The following is a stepbystep procedure to working dilution problems, and includes. In microbiology, serial dilutions log dilutions are used to. Karla fjeld demonstrates how to perform serial dilutions using microbiologics lyophilized qc microorganism pellets. A dilution is prepared by adding a specific amount of a concentrate to water to obtain a new volume and concentration. Solutions can be diluted by removing a known amount from a solution of known concentration and mixing it with an amount of solvent to reestablish the original volume. Acids and bases usually come in concentrated form, as concentrates, but they are rarely used in this form. Molarity, dilutions, and preparing solutions lab r. In this lab, a serial dilution will be made of a sample of bacteria, and then those dilutions will be used to.
Serial dilutions are useful in a small quantity of serum and to find the titer of antibodies. This exercise is intended to familiarize us with using scientific notation to express big and small number easily and the process of using serial dilutions to make concentrations of known values. Lab 9 serial dilution, pour plates, and enumeration of bacteria introduction. Molarity and serial dilutions teacher handout module overview one of the most important skills in science is to calculate dilutions. Serial dilution to begin, you must know how to calculate dilution. To make our dilution we would use 200ul of serum and 200 ul, or 800 ul of water.
Typically, the dilution factor remains constant for each dilution, resulting in an exponential decrease in concentration. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then being a 110 dilution of the previous one in the series. Standard bacterial plate count uncontrolled copy 3. The dilution error although present in the serial dilution experiment is not an input. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. Using a turntable and sterile bent glass rod see fig. We are often concerned with how much solute is dissolved in a given amount of solution. Solutions part 1 solutions used in clinical laboratory. The instrument we are going to run this test on needs at least 1 mlul of fluid to run the test so how shall we make this dilution. In this lab you will investigate several techniques for working with solutions. As shown in the illustration below, it begins when 1 ml of the bacterial sample is added to 9 ml. The wellpro 4000ce is the latest addition to the wellpro line of instruments. You find out that the lake was closed because of a health hazard.
Received 9 may 2014 received in revised form 29 august 2014. For example, when you are making orange juice from a frozen concentrate, you mix 1 can of concentrate with 3 cans of water. How to perform serial dilutions in microbiology dr. This exercise is intended to familiarize us with using scientific notation to express big and small number easily and the process of using serial dilutions to make. Serial dilution simulation lab the american biology teacher. How to do a serial dilution 9ml test tubes mccc microbiology. We will begin our discussion of solution concentration with two related and relative terms dilute and concentrated. Serial dilutions involve diluting a stock or standard solution multiple times in a row. Davidsonb a rdecom, edgewood chemical biological center, aberdeen proving ground, md 21010, usa b science and technology corporation, belcamp, md 21017, usa article info abstract article history. Estimation method for serial dilution experiments avishai bendavid a. While you may already make solutions in the lab by following recipes, we hope. Perform plate filling, serial dilutions and plate replication by column or by row in 96 or 384 well plates. Serial dilutions are often performed when titering antibodies or when generating amplified dilutions of an analyte.
When preparing to perform serial dilutions, there are three main components to. The first dilution is made just like the simple dilution. Calculate mathematical manipulations and problems for basic math, the metric system, and dilutions. Each step in this dilution uses one ml of the solution prepared in the previous step, which is added to nine ml of water. In biology lab work, concentrations are often given in micromoles or m. Molarity of solutions pdf dilution of solutions pdf ii pdf molarity and stoichiometry pdf colligative properties pdf textbook problems pdf article hot and cold packs chemmatters feb. Dilution lab dilutions part 2 of 4 serial dilutions dilutions. Serial dilution is often a difficult concept for students to understand.
What does this mean if a solution has a 110 dilution the number represents 1 part of the patient sample added to 9 parts of diluent. Jan 15, 2019 explain how concentrations can be changed in the lab. Serial dilution lab in this unit we will be discussing measurements of concentration that us e both very big and very small numbers. How to dilute a solution this video takes you through the procedure for diluting a solution. The highly concentrated solution is typically referred to as the stock solution. Background serial dilution is an important technique in many areas of experimental biology, and working with. In order to calculate dilutions of solutions the equation. In chapter 5 we briefly described the use of a 3fold serial dilution scheme for inhibitor titrations to create concentrationresponse. In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Protocol for using serial dilutions in the microbiology lab. This results in a solution of a molarity proportional to the amount removed divided by the total volume. Dilution and concentration theory dilution and concentration theory sounds ominous but you use dilutions and concentrations fairly frequently outside of your scientific life.
This webinar is intended to give a brief introduction into the mathematics of making solutions commonly used in a research setting. Lab math solutions, dilutions, concentrations and molarity. In this short dry lab exercise, students perform serial dilutions using seed beads. Clinical lab dilutionsmlt 241 intro to clinical chemistry. Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions. For example, a tenfold serial dilution could result in the following concentrations. Label 2 or 3 refer to the firms outline of production op plates per dilution series for each serial.
After diluting by the same dilution factor several times a serial dilution, the dye will seem to disappear. Investigating the mathematics of serial dilutions exploration two interested in the effect of dilution on the concentration of a solution, a chemist compiles the data in table 3 below outlining a different type of dilution. Word definition concentration the amount of a substance solute dissolved in a liquid solvent in a chemical solution. The importance of serial dilution and colony counting is reflected by the number of. Jan 27, 2020 another common dilution problem involves deciding how much of a highly concentrated solution is required to make a desired quantity of solution of lesser concentration. This is defined as multiple progressive dilutions ranging from a more concentrated solution to a less concentrated solution. To determine the concentration of a cleaning solution that will kill fifty percent of a given population of tubifex lc 50. However, the numbers of microbes in a given specimen are required to identify in particular aspect such as diary manufacturing, diseases research and study, and.
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